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Complement Antigen Technology

Test Methodology

My test methodology differs significantly from all others. I was granted a patent for its unique nature. It is highly reproducible and clinically relevant. The plates are coated with 44 different allergens (food, food additive or dyes) arranged in a micro titer array. The antigens we use are those most often eaten in the American diet.

My unique test employs a three-point standard curve. We establish a true standard calibration of each plate that validates the accuracy of the readings we obtain, and verifies each antigen test result for each individual. The standard curve generates a cut off point which allows for the variation from individual to individual. The food antigen and IgG antibody bind to each other and fix complement. Forty-four food antigens are bound to wells in a plate so that they are non-reactive until a patient's serum is introduced. Specific binding of IgG and Components of Complement (identifying immune complexes) to specific foods are identified by monoclonal antibodies.

A patient's serum is added to the plate and their antibodies to both IgG and components of complement (analyte) bind to the allergen(s). The plate is washed and an enzyme conjugate is added that recognizes the bound antibodies of both IgG and immune complexes. After incubation and washing, substrate is added to visualize the bound antibodies of both IgG and components of complement (immune complexes). The amount of optical density is proportional to the amount of bound antibody to IgG and immune complex. A report depicting these reactions is plotted as a simple bar graph which is easy to interpret.

Using this patented method we can state with 80% clinical accuracy that a patient has a positive or negative reaction to a particular antigen. Research has shown a two-fold increase in clinical relevance with this technique.

 Complement Antigen Technology - Hypersensitivity Reactions Complement Antigen Technology -
Hypersensitivity Reactions