Sage Test Technology

Sage Test Methodology

The Sage Test Methodology differs very significantly from all others. It is highly reproducible and clinically relevant. Sage's ELISA plates are coated with 44 different allergens (food, food additive or dye causing allergies) in duplicate arranged in a micro titer array. The antigens of interest are those consumed in the common American diet. Our unique test employs a three-point standard curve. We establish a true standard calibration of each plate that validates the accuracy of the readings we obtained, and verifies each antigen test result for each individual. The standard curve generates a cut off point which allows for the variation from individual to individual. The food antigen and IgG antibody bind to each other and fix complement. Forty-four food antigens are bound to wells in a 96-well plate so that they are non-reactive until a patient's serum is introduced. Specific binding of IgG and Immune complexes to specific foods are identified by a monoclonal antibodies.

A patient's serum is added to the plate and their antibodies to both IgG and immune complexes (analyte) bind to the allergen(s). The plate is washed and an enzyme conjugate is added that recognizes the bound antibodies of both IgG and immune complexes. After incubation and washing, substrate is added to visualize the bound antibodies of both IgG and immune complexes. The amount of optical density is proportional to the amount of bound antibody to IgG and immune complex. A report depicting these reactions is plotted as a simple bar graph which is easy to interpret. Using this method we can state with 95% accuracy that a patient has a positive or negative reaction against a particular food. This methodology is unique to our test for delayed hypersensitivity. Research has shown a two-fold increase in clinical relevance with this technique.

Sage Test Technology - Hypersensitivity Reactions Sage Test Technology - Hypersensitivity Reactions